Entering edit mode
                    11.1 years ago
        Arthur Melo
        
    
        ▴
    
    10
    Dears, I'm running Picard to MarkDuplicates in a _merged.sorted.bam file from RNA-seq data. My script is:
java -Xmx5g \
  -jar /usr/local/picard-tools-1.119/MarkDuplicates.jar \
  INPUT=merged.sorted.bam \
  OUTPUT= merged.sorted.deduped.bam \
  METRICS_FILE=metrics_duplicates.txt \
  REMOVE_DUPLICATES=TRUE \
  VALIDATION_STRINGENCY=LENIENT \
  ASSUME_SORTED=TRUE \
  CREATE_INDEX=TRUE
So, a strange message show:
OpenJDK 64-Bit Server VM warning: You have loaded library /usr/local/picard-tools-1.119/libIntelDeflater.so which might have disabled stack guard. The VM will try to fix the stack guard now.
It's highly recommended that you fix the library with 'execstack -c <libfile>', or link it with '-z noexecstack'.
I don't have idea about this problem...
I would be extremely grateful to some help.
Thank you.
Hello arthurmelobio!
It appears that your post has been cross-posted to another site: SEQanswers.
This is typically not recommended as it runs the risk of annoying people in both communities.