Hello All, I want to see differential expression of an individual gene (a single RNA binding protein), in different cancer vs normal conditions and further look into expression correlation with other splicing transcripts that are targets of this gene.
I want to work with level 3 data, i'm biologist and new to RNASeq. but i understand tools like DESeq 2, EdgeR are recommended. so is it possible to work without using R/bioconductor packages for DEG analysis ? and how should i proceed.