Question: (Closed) What's the best way of generating high yields of mitochondrial DNA
0
gravatar for Yongjie Zhang
3.9 years ago by
UC Berkeley, USA/ Shanxi Univ, China
Yongjie Zhang80 wrote:

Hello,

I want to extract mitochondrial DNA from a fungus, but there are two ways to cultivate the fungus, one is to cultivate it on an agar medium (covering a piece of cellophane paper to stop hyphae to grow into the medium) in a petri dish, and the other is to cultivate it in a liquid medium in an erlenmeyer flask (shaking during cultivation). Do you know, between the two ways, which is better for getting high yeilds of mitochondrion and mitochodnrial DNA?

I ask because I ever used the first way to cultivate and performed RNA-seq, but the number of mitochondrial reads only accounted for 0.05% of total reads. I expect at least 0.5%. So, I'm not sure if the second way of cultivation would help me.

Any comments are appreciated.

Yongjie

mitochondrial dna • 726 views
ADD COMMENTlink written 3.9 years ago by Yongjie Zhang80

This question would be more appropriate over at biology stackexachange.

ADD REPLYlink modified 3.9 years ago • written 3.9 years ago by genomax84k

Hello Yongjie Zhang!

We believe that this post does not fit the main topic of this site.

not related to bioinformatics

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

If you disagree please tell us why in a reply below, we'll be happy to talk about it.

Cheers!

ADD REPLYlink written 3.9 years ago by Pierre Lindenbaum128k
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