Use MEGAN6 to classify functional genes

Question

[wangli_lily]

Dear All,

I used MEGAN6 to analyze my PE reads from Illumina. After blastx reads with nr database (outfmt 0), the results was imported into MEGAN6. And I do click "taxonomy", "SEED", and "KEGG" analysis. However, the taxonomy tree showed well, but all the reads were assigned as "no hits" under both seed and kegg wingdows. I also tried to load the gi mappling file during the importing step, but the problems did not change. Can anyone help to figure it out?

Thanks!

[h.mon]

Can MEGAN import blastx -outfmt 0? I think you have to use -outfmt 6, or xml.