Question: Genome coverage with pool of individuals
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gravatar for jomaco
2.2 years ago by
jomaco180
European Union
jomaco180 wrote:

If I have:

  • Pooled DNA from multiple different but genetically related (sibling) individuals, sequenced in one sequencing run
  • DNA from another individual (genetically related (sibling) to those in the pool), sequenced on its own

Then If I assemble the single individual and map the pooled data to it, would I see greater (depth and breadth) of coverage across the genome assembly for the pool (assuming normalisation between read libraries) than if I were to sequence just one of those individuals from the pool and map that data to the assembly? (i.e. the pooled reads are more likely to cover the regions of the genome covered by the assembly because there's a pool of individuals)

Is there a biological reason for obtaining a slightly different representation of the genome for each of those individuals in the pool - for example, that would confound DNA extraction in a specific way for each individual such that coverage is affected?

Thanks

pool genome coverage • 693 views
ADD COMMENTlink modified 2.2 years ago • written 2.2 years ago by jomaco180

I read it twice and I'm not sure I understand what you mean.

ADD REPLYlink written 2.2 years ago by WouterDeCoster32k
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