Hello all i have a Sanger read (an *.ab1 file which i did basecalling for it) and a reference. i want to find homo and hetero events for this sample (read) for further computations like annotating individual variants. now i did it myself but it seems there is a standard pipeline for this purpose for NGS reads. is there any pipeline for sanger reads as well? or i need to convert my read into fastq for this purpose? and what if "yes" what is the pipeline? i'm using Rstudio Bioconductor.