I'm hoping to find a low-cost alternative to the ERCC spike-in controls for use in qRTPCR. Due to a high concentration of lipids in my tissues, RNA extraction between samples can be variable. I'm hoping to use something like the ERCC or TATTAA spike-in controls to normalize differences between sample prep. Ideally, I would use 3-5 synthetic RNA oligos to spike in.
Both the commercial spike-ins are pretty pricey, and more extensive than what I need. Has anyone had success with their own do-it-yourself (DIY) spike-ins? Did you buy synthetic RNA oligos from a company and then poly(A)-tail and 5'-cap them yourself? Are there companies that will do the tailing and capping in addition to oligo synthesis?
Thanks for any advice!