Dear Friends, Hi ( I'm not native in English so, be ready for some possible language flaws).
I have done DEG analysis on de novo assembly created via Trinity software.
Now I want to pull out my DEG sequences for annotation, and I have created two collection.fasta files containing the ID names of those DEGs (one for Male sample DEGs and one for female sample DEGs)
but the DEG result IDs is as: TRINITY_DN212724_c0_g1 and the structure of Trinity.fasta is as TRINITY_DN212724_c0_g1_i# and it is usual that each gene has several isoformes.
I have used a program called "faSomeRecords" but it seems that it could not collect all the isoform of a gene ID automatically.
I have add "_i* " at the end of all gene IDs in the DEG files, but no chance !
I need a tool to collect all isoforms of some selected genes (collection.fasta file) from Trinity.fasta file, please.
Thank you in advance