Question: GWAS with few samples
0
gravatar for LNA
3.1 years ago by
LNA30
LNA30 wrote:

Hello,

I have SNP data of a Illumina HumanOmni5-4 BeadChip (4.2 million SNPs) for 28 samples with a quantitative trait. I have no experience doing GWAS studies and I need some advice. So far, I performed the quality control steps suggested by the GWASTools Vignette.

Which testing procedure is best for this kind of data? It seems, such a small number of samples leads to no significant p-values. Any way to improve this? Should I use the quantitative trait directly with linear regression based testing or does it make any sense to group the samples and do a case-control study?

Any suggestion would be of great help to me!

Thanks, Lna

snp gwas • 1.1k views
ADD COMMENTlink modified 3.1 years ago by coleman_jonathan420 • written 3.1 years ago by LNA30
1

Unless your effect size is extremely huge, 28 samples will never be sufficient to get significant/meaningful and accurate association results.

ADD REPLYlink written 3.1 years ago by WouterDeCoster41k

I have to analyze that data. Since increasing the sample size is not an option, I want to know what is the best I can do?

ADD REPLYlink written 3.1 years ago by LNA30
0
gravatar for coleman_jonathan
3.1 years ago by
European Union
coleman_jonathan420 wrote:

Assuming your QT is a genuine continuous variable, you should use the QT. It has greater power (read this: http://www.ncbi.nlm.nih.gov/pubmed/11987478)

ADD COMMENTlink written 3.1 years ago by coleman_jonathan420
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