I'm creating a protein protein interaction network in STRING (http://string-db.org/) with 110 proteins, but appeared some doubts.
1. What "minimum required score interaction" use?
I made my network with> 0.400, but found papers that use> 0.700, already one with> 0.150.
Ai came the question, what "minimum required interaction score" should I use?
2. Use or not use the "textmining"?
I saw in some papers, researchers removing this option.
Ai came the question, what options I should keep?
3. Can I do search only one protein in string (max number of interactors to show: 1st shell: no more 10) and then do the merge in Cytoscape with the network? That is correct? or was manipulating my data?
The aim is to know the interactions of each of the 110 proteins and how they interact with each other and so on.