I'm currently diving into isoforms differential expression analysis results. I use the duo RSem-EBSeq to perform this work. I'd like to build a volcano plot of my data.
RSem-EBseq returns posterior probabilities (PP) and no p-values (PV) at all. I am aware, that both PP and PV allows me to answer the same question. I know that PP goes from 0 the lowest probability to 1, the highest. So I could just multiply those values b -1 to have a volcano plot in the same orientation as a "classic one".
Is it acceptable to do such a thing ?