Question: Finding the transcript direction using stranded-specific library
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gravatar for seta
2.6 years ago by
seta1.1k
Sweden
seta1.1k wrote:

Hi all,

I have paired-end stranded-specific library from Illumina sequencing, which assembled (de novo assembly) using Trinity. As I used the stranded-specific library, could you please let me know how I can determine the direction of contigs (transcripts) withing the assembly?

Thanks in advance

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