Finding the transcript direction using stranded-specific library
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7.5 years ago
seta ★ 1.9k

Hi all,

I have paired-end stranded-specific library from Illumina sequencing, which assembled (de novo assembly) using Trinity. As I used the stranded-specific library, could you please let me know how I can determine the direction of contigs (transcripts) withing the assembly?

Thanks in advance

RNA-seq stranded-specific assembly • 1.4k views
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