Entering edit mode
7.5 years ago
igor
13k
I have a long sequence (1-5 MB). I am trying to BLAST it using web-based NCBI BLAST against the nt database to see if it's a known organism. It works with megablast (highly similar sequences), but the results aren't great. If I try discontiguous megablast or blastn, it crashes. Is that a known issue? Would running BLAST locally solve that? Is there an alternate solution (I would prefer not to download the entire nt database)?