Hi all friends,
I'm working on a RNA-seq project of a non-model plant, the library is generated from mRNA fraction (enriched with polyA) and sequenced as PE, stranded-specific. I have done de novo transcriptome assembly and annotation. Now, I would like to know if there is any way to determine the 3 and 5 UTR region of genes? Regarding 3 UTR, Since the library enrichment was done by oligo-dT primers, I'm not concerned about it, but I don't know what is the right procedure to determine these regions? Could you please advise me on this issue?
Thanks in advance