Entering edit mode
7.5 years ago
yanayatskiv1996
•
0
Hi!I would like to analyze a codon structure of a set of human coding sequences. To do this, I first need to create a daset - as many as possibleCDS variants of a single human gene from different individuals. What would be the simplest way to do so?
Essentially you would first need raw RNA-sequencing data, which you can find on SRA.
I cant find it for my gene.
What have you tried?
I tried to find here: http://dl2.joxi.net/drive/2016/10/24/0018/0152/1183896/96/e22e731680.jpg
Assuming you searched SRA for your gene of interest: that's not how you should use it. In SRA entire experiment datasets are deposited, e.g. all reads from entire mRNA sequencing. So you have to find a tissue in which your gene is expressed in which sufficient samples were sequenced, download the data, process it, isolate the part you are interested in.
Oh, I'm only a beginner so i dont know how it works. Thank you for your help. I will try.