What is a different isoform in Flybase?

Question

[skmook16]

When I find genes from Flybase, some genes have a couple isoforms (transcript variants). I wonder how they are generated. Alternative splicing or alternative promoter? For example, I searched a MDR49 gene and there are two different isoforms. They are generated by alternative splicing or alternative promoter?

Thanks!!

Answer 1

[natasha.sernova]

See this link:

http://flybase.org/static_pages/newhelp/gbrowse_geneModels_evidence_help.html

and "allows prediction of alternatively spliced isoforms" in it.

The whole paragraph:

"NCBI gnomon Gene prediction (coding region of transcript) generated via a hidden Markov model using transcript alignment constraints and protein hit information, if available; allows prediction of alternatively spliced isoforms (Souvorov, et al., 2006, NCBI); submitted by J. Ostell. "

http://flybase.org/reports/FBgn0259246.html

In the upper right corner replace :"Jump to gene" to your preferred gene-name and press GO:

You will get this page:

http://flybase.org/reports/FBgn0004512.html

Again on the left you can play with "gene region": intron, exons, etc.