Dear all Hi,

I would like to know if I can apply anova to my log2 transformed data.

We have SNV + INDEL mutation count data for ICGC data and we trying to prove in specific regions Breast Cancer have higher rate of mutations. But because the total number of mutations in data is too dispersed we apply log2 transformation. Therefore I would like to know if is it okay to apply anova in this case because all the mean and variance calculations are different in log2 scale.

My aim is to prove the means are not same across cancers then post hoc test to perform individual comparisons with t.test.

Best regards,

Tunc.

ANOVA has three assumptions (this is from wiki!):

If your log2 transformed data comply with these assumptions, you can try ANOVA.