This is a simple formula used to calculate the temperature ＞＞ Tm = 2(A+T) + 4(G+C) ℃
I curious about the Nearest-neighbor method .
I get the deltaH and deltaS but when I put this 2 parameter in the formula I got a very small numer than the correct answer .
Can someone teach me how to count ?
this is my primer : GCTAGTTCAACGTCAAGTGTG
Enthalpy : -167 cal/mol
Entropy : -455.9 cal/mol-K
I use this fumula
Tm = delta H / (delta S + 1.99 x ( ln(c/4)) - 273.15 = 87.14 C
Tm = (-167 ) / ( -455.9 + 1.99 x ln(c/4)) - 273.15 = －272.8
where c is probe concentration. In this case 0.1
Can someone teach me how to count ? thx!