I would like to ask you; which one or ones are more important to identify homolog sequences using blastp?
Identify % ?
I am trying to find homolog sequences of candidate neuropeptides in my database by searching with blast p. I have an output (format 6). I want to filter results, then I will search in Uniprot o blast to identify my candidate proteins as neuropeptides or not. For this, which parameter should I use? Evalue, bit score, identify %, or combine them?
my approach will be to filter sequences which have e-value lower than 0.01 and bit score bigger than 90.
is this reasonable ? or is it possible to miss some neuropeptides.?