I'm experimenting with Minia for genome assembly of a eukaryote with a 500 MB genome size. I've had good luck adjusting the parameters to find the optimum, except for one: estimated_genome_size.
The manual suggests this: estimated_genome_size
And I have added this to the command line: -estimated_genome_size 500000000
With and without preceeding dashes, changing underscores to dashes, etc, but always get this result: ERROR: Unknown parameter '-estimated_genome_size' ERROR: Unknown parameter '540000000'
Further, the Minia options which are displayed as the result of the error do not include estimated_genome_size. Is this parameter no longer recognized, or does anyone have any suggestions?
Thanks for your help.