I am trying to run the script "normalize-by-median.py" of the khmer software but always I got this error message: "ERROR: Unpaired reads when require_paired is set!"
My question is: Do I need to merge the two files with the forward and reverse reads in one file? or the software can use each file individually and internally merge them? If the latter is the response, how I can introduce both files to the software? (In the instructions on the web I read only the way for one file)
I ran some examples but with my reads it does not work
Thank you very much for your help