CUFFMERGE from many assemblies to define transcripts but only compare a subset?
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4.3 years ago
bkafsack • 0

I have RNAseq assemblies for the same organism from multiple different experiments. Let's say I have experiments A, B, and C each with conditions 1 and 2.

Under the assumption that exon usage doesn't vary between experiments, can I use cuffmerge on all the assemblies to get a better overall model of transcripts before only comparing a subset of assemblies with cuffdiff? Or will this throw off the statistics in the subsequence cuffdiff steps?

So can I run cuffmerge on A1,A2, B1, B2, C1, C2, followed by cuffdiff on only C1 & C2?

Thank you in advance for your input.

RNA-Seq cufflinks tuxedo cuffmerge cuffdiff • 974 views
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4.3 years ago
Satyajeet Khare ★ 1.6k

Yes, cuffmerge on all samples should be fine.

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