I have been trying to use BWA-MEM in a AWS Cloudman cluster to align paired end reads (150bp) from an NGS run. Each read file is ~130 GB so I am estimating the resulting BAM will be ~100 GB. I have configured the cluster a couple of different ways but the mapping just crawls along and I am terminating it after only a few gigs because the AWS costs start spiraling. Anyone have any recommendations on either how to configure an efficient cluster for this alignment? Any other good options to map using AWS/EC2 environment? Thanks!
Question: Help configuring Galaxy Cloudman for mapping
3.2 years ago by
KJ Kunz • 0
KJ Kunz • 0 wrote:
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