Error with blastn on windows

Question

[laila.adel]

Dear all, I ran this command to do blastn (the formatdb ran well, it didn't give me errors):

blastall.exe -p blastn -i Fosmid_DNA_scaffolds.fasta -d CC2_Fosmid_sequence -o Fosmid_DNA_scaffolds.fasta.vs.CC2_Fosmid_sequence.blastn -b 1000 -v 1000 -F "m S" -e 10 -a 4

But I got this error:

[blastall 2.2.26] ERROR:  SBlastFilterOptionsValidate: SEG filtering is not supported with blastn
[blastall 2.2.26] ERROR: scaffold1: Failure at filtering

If possible, kindly tell me what is the problem? Is it the code? Or how to avoid this error & run it properly?

Thanks!

Laila Ziko, MSc

[GenoMax]

I don't recall what the -F option is. Have you tried to remove that and see if blast works? This appears to be an older version of blast (current is v.2.5).