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7.3 years ago
Bioinfonext
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460
I am trying to understand how can be read a particular locus on both chromosomes when we do sequencing, and calling a particular SNP homozygous or heterozygous.
Like for SNP detection within a DNA againt the reference sequence:
1) We are doing fragmentation of DNA 2) Ligation of adapter and bar code 3) PCR 4) Denaturation 5) Sequencing
So how we can determine that both chromosome segments were sequenced for a particular locus?