Hoping that some of you experienced BUSCO users can help me:
What I did:
- Using the virtualbox (ubuntu) for BUSCO on MacOS (BUSCO v2 using Ubuntu virtual machine, with all dependencies included from the BUSCO webpage)
- Downloaded my fasta file trinity.fa (with wget) from dropbox to the virtual machine and saved it in the "busco2" under a folder called "MyProject1" (created by mdir). I have opened the file, and it seems fine.
- Downloaded the eukaryota_odb9 lineage and extracted it in the lineage folder under BUSCOVM
- wrote in the ubuntu terminal: python BUSCO.py -i trinity.fa -o transcriptome -l eukaryota_odb9 -m trans
What happened: ERROR Impossible to read trinity.fa
Does anyone know how to overcome this error?