zero TMM : assembly error or biologically important phenomenon?
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7.5 years ago
Farbod ★ 3.4k

Dear Biostars, Hi

I have some transcripts (RNA-seq/ illumina/ Trinity de novo assembly/RSEM) TMM values that are zero for 3 replications of condition 1 (C1) and two of condition 2 (C2) .

Does these transcriptes worth for further analysis and investigation or not?

Example :

C1a-TMM......C1b-TMM......C1c-TMM.................C2a-TMM........C2b-TMM.......C2c-TMM

....0..................0...................0.............................0.......................0.75............0.........

~ Best

RNA-Seq gene • 1.4k views
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