how to use -u/--multi-read-correct and -b/--frag-bias-correct in my command?
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7.3 years ago
PK ▴ 130

In my cufflinks_2_0_2 i have to use -u parameter and -b parameter. how do i use? i have used this command

/opt/cufflinks_2_0_2/cufflinks -p 8 -o condition -u -b --GTF ~/ACTREC/annotation/genes.gtf ~/ACTREC/Tophat/Tophat_P10_Rep1/accepted_hits.bam
(for your understanding i have highlighted. What i need to mention?)

software error RNA-Seq ngs • 1.5k views
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Did you get an error message?

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Thanks for your reply, No i didn't get any error but i want to know did i anything wrong. Actually i was confused -u default is FALSE but here i gave .gtf file it is correct?

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