error in bowtie 2
0
1
Entering edit mode
7.2 years ago
mra8187 ▴ 20

Dear all When I use bowtie 2 i get this error What can I do ?

(ERR): bowtie2-align exited with value 137

RNA-Seq • 2.0k views
ADD COMMENT
0
Entering edit mode

Could you provide some more details,like the command you gave etc..

ADD REPLY
0
Entering edit mode

In addition to what Jeffin Rockey said, since you tagged this as "rna-seq", you should be warned that bowtie2 is not appropriate for RNAseq data. Use STAR or something similar instead.

ADD REPLY
0
Entering edit mode

Unless it's prokaryotic RNA-seq?

ADD REPLY
0
Entering edit mode

Yes its RNA seq from cow ...

ADD REPLY
0
Entering edit mode

Please use ADD REPLY to answer to earlier comments, as such this thread remains logically structured and easy to follow.

For RNA-seq alignment of eukaryotic genomes you need a spliced aligner, most recommended nowadays are STAR and HISAT2.

ADD REPLY

Login before adding your answer.

Traffic: 853 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6