Errors when re-importing an alignment back to Mega
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6.1 years ago
genya35 ▴ 40

Hello,

I would really appreciate if someone could advise on the following error with Mega7 GUI version.

I'm experiencing errors when trying to re-import alignments back into Mega. I performed ClustalW alignment on 400 protein sequences and exported in .meg format. However, when I try to open the file by either right clicking on the file or opening it via "Retrieve sequences from a file" I get 'Invalid base found >: in line 12343". It doesn't make sense because the alignment was perform through the tool and saved in mega format. Could some one please advise how to fix the error so I can re-import the file?

Thanks

alignment software error • 4.5k views
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Just to clarify order of events. You did a MSA in MEGA7 using clustalW and saved the project a .meg file? Now you are not able to re-open that file (File --> Open a file/session --> Select the .meg file)?

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I ran the alignment today and clicked on "Data/Phylogenetic Analysis". It opened a Text file editor and displayed an error message "Invalid base found" and it goes to the end on the alignment were there are no bases. This happens even before I export the data. The same error occurs when I import the alignment back into Mega. How can I fix this?

Thanks