I would really appreciate if someone could advise on the following error with Mega7 GUI version.
I'm experiencing errors when trying to re-import alignments back into Mega. I performed ClustalW alignment on 400 protein sequences and exported in .meg format. However, when I try to open the file by either right clicking on the file or opening it via "Retrieve sequences from a file" I get 'Invalid base found >: in line 12343". It doesn't make sense because the alignment was perform through the tool and saved in mega format. Could some one please advise how to fix the error so I can re-import the file?
Just to clarify order of events. You did a MSA in MEGA7 using clustalW and saved the project a
.megfile? Now you are not able to re-open that file (
File --> Open a file/session --> Select the .meg file)?
I ran the alignment today and clicked on "Data/Phylogenetic Analysis". It opened a Text file editor and displayed an error message "Invalid base found" and it goes to the end on the alignment were there are no bases. This happens even before I export the data. The same error occurs when I import the alignment back into Mega. How can I fix this?