Question: TFBS ChIP-Seq: No Cross-correlation Peaks other than Read Length: Is it normal?
0
gravatar for MMa
3.9 years ago by
MMa290
MMa290 wrote:

I'm currently analyzing someone else's ChIP-seq data, and noticed after routine mapping and filtering, there's only one real peak from the cross-correlation plot generated by phantompeakqualtools at read length. While PPQ tools also called other peaks, they're obviously artifacts. NSC is within normal range (5-10), RSC is low (0.1-0.3), but this can be excused by the low expected numbers of TFBS.

In this case, is it appropriate to go on with peak calling?

chip-seq • 1.1k views
ADD COMMENTlink modified 3.9 years ago by Devon Ryan98k • written 3.9 years ago by MMa290
1
gravatar for Devon Ryan
3.9 years ago by
Devon Ryan98k
Freiburg, Germany
Devon Ryan98k wrote:

NSC/RSC and strand cross-correlation are mostly meaningless, don't base anything off of them (or even bother calculating them). A more useful thing to do is look at a "fingerprint" plot (e.g., from plotFingerprint in deepTools), though really you're going to do peak calling anyway regardless of what the results are, since that's quick and repeating experiments takes a while.

ADD COMMENTlink written 3.9 years ago by Devon Ryan98k
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