Does MACS2 or MACS14 perform total reads normalization?
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8.5 years ago
bxia ▴ 180

Does MACS2 or MACS14 perform total reads normalization?

I didn't see them to discuss about it. Just want to make sure the default parameters doesn't do the total reads normalization.

Thanks,

ChIP-Seq • 2.4k views
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The chip and control reads are scaled to same sequencing depth ( towards lower ) before doing a poisson test for signal enrichment.

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