Question: long non-coding RNA in genome guided assembly
0
gravatar for nabiyogesh
9 weeks ago by
nabiyogesh50
South Korea
nabiyogesh50 wrote:

I have mapped pair end Illumina read to genome using Hisat2 and further assembled into transcripts using StringTie. I am wondering whether these assembled transcripts contains long non-coding RNA or not.

If Yes, then please suggest how can I remove these long non-coding RNA.

Thanks in advance.

rna-seq • 106 views
ADD COMMENTlink modified 9 weeks ago by liuwei0 • written 9 weeks ago by nabiyogesh50
0
gravatar for liuwei
9 weeks ago by
liuwei0
Shanghai
liuwei0 wrote:

When you use StringTie to asemble, the gtf file contain mRNA and non coding RNA.So,filt the non coding RNA in the gtf file and the result will only be protein coding transcrpt.

ADD COMMENTlink written 9 weeks ago by liuwei0

Please suggest the tool that can remove non-coding RNA from gtf file.

ADD REPLYlink written 9 weeks ago by nabiyogesh50

you should understand the feature of GTF file and use shell command can to that( google 'shell grep')

ADD REPLYlink written 9 weeks ago by liuwei0
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