Question: RNA Sequencing Trimmomatic
2.0 years ago by
neeshatripathi87 • 0
neeshatripathi87 • 0 wrote:
Hello all, I have all my like 10-15 fastq file in 1 folder named combined and I need to trim that combined file. I tried some of the command like
TrimmomaticPE -phred33 /R1_001.fastq.gz /R2_001.fastq.gz /R1_pairedout /R1_unpairedout /R2_pairedout /R2_unpairedout ILLUMINACLIP:/TruSeq3-PE.fa:2:30:10 LEADING:5 TRAILING:5 AVGQUAL:20
but that didn't work. Please give me some idea to trim.
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