Agilent focused exome panel public example fastq data
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7.1 years ago
abdulbenissa ▴ 10

Hello, Everyone !!

I was looking for a source to obtain FASTq files for the panel focused exome panel from agilent. I had to use https://earray.chem.agilent.com/suredesign/home.htm to obtain the bed files but FASTA to FASTq is ideal for me. I am testing the pipeline we have before buying the kit !!

any information is valuable

Thanks in advance

next-gen sequencing gene SureSelect • 2.2k views
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are you talking of fastq ? fastqc is a tool for quality assessment of fastq

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Put your reply in comment as it's not an answer. To convert fasta to fastq take a look at this link. Make sure that you have received fasta file with QUAL scores since fastq files contain the quality scores. Ideally if you are using sure select which are become enrichment bed files for target enrichment then you usually use them as bed files in pipelines to understand on target enrichment.

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sorry, I fixed it , I am looking for fastq files generated from SureSelect Focused exome.

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Please use ADD COMMENT or ADD REPLY to answer to previous reactions, as such this thread remains logically structured and easy to follow. I have now moved your post but as you can see it's not optimal. Adding an answer should only be used for providing a solution to the question asked.

I also modified your title to better reflect what you are asking for if my interpretation is correct.

Ontopic: have you tried asking agilent for example data? Sure they have tested their kit, no?

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