Question: (Closed) how to design primers for this pseudogene (AC112229.1) that not amplified his parental gene or another isoform ?
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gravatar for reimco2
2.1 years ago by
reimco20
reimco20 wrote:

Hi every one, I'm trying to design primers for this pseudogene (AC112229.1). But these pseudogene are not available in nbci nucleotide it's take a part of BAC, and his parental gene is (ZNF532). when i toke the sequence of pesudogene and design primers. most primers cover or amplified the parental gene and others isoforms and not pseudogene. I need to desing primers for Real-time qPCR( RT-qPCR) and my target is quantify pseudogene expression. How to design primers in this case. Hope to hear advices from you.. Thanks a lot.

sequence tutorial • 728 views
ADD COMMENTlink modified 2.1 years ago by Giovanni M Dall'Olio26k • written 2.1 years ago by reimco20

Have you tried https://www.ncbi.nlm.nih.gov/tools/primer-blast/

ADD REPLYlink written 2.1 years ago by Sej Modha4.2k

Hello reimco2!

Questions similar to yours can already be found at:

We have closed your question to allow us to keep similar content in the same thread.

If you disagree with this please tell us why in a reply below. We'll be happy to talk about it.

Cheers!

PS: Please try to improve your previous question.
ADD REPLYlink written 2.1 years ago by Michael Dondrup46k
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