Question: (Closed) how to design primers for this pseudogene (AC112229.1) that not amplified his parental gene or another isoform ?
gravatar for reimco2
3.5 years ago by
reimco20 wrote:

Hi every one, I'm trying to design primers for this pseudogene (AC112229.1). But these pseudogene are not available in nbci nucleotide it's take a part of BAC, and his parental gene is (ZNF532). when i toke the sequence of pesudogene and design primers. most primers cover or amplified the parental gene and others isoforms and not pseudogene. I need to desing primers for Real-time qPCR( RT-qPCR) and my target is quantify pseudogene expression. How to design primers in this case. Hope to hear advices from you.. Thanks a lot.

sequence tutorial • 1.2k views
ADD COMMENTlink modified 3.5 years ago by Giovanni M Dall'Olio27k • written 3.5 years ago by reimco20

Have you tried

ADD REPLYlink written 3.5 years ago by Sej Modha4.7k

Hello reimco2!

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ADD REPLYlink written 3.5 years ago by Michael Dondrup47k
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