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6.9 years ago
1374906590
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Hello, I want to assemble transcripts from tophat's result, but when I run this command:
stringtie ./tophat_out/accepted_hits.bam -p 16 -G Amborella_trichopoda.AMTR1.0.35.gtf -B -o stout/transcripts.gtf
it reported the error:
GFF Error: overlapping duplicate transcript feature (ID=AMTR_s00001t00279210)
I used a annotation file with GTF format where there isn't a line with "ID=AMTR_s00001t00279210".I don't know what's wrong with it. What should I do? Thank you very much