I have a human RNA-SEQ project consists 4 samples; 2 condition each of them has 2 replicates. Three of four samples contain 20 millions paired-end reads however one of them consist 50 millions paired-end reads. Read length is same for all samples, 150bp.
I'd like to know, Is this reads difference will affect on my final result or not?
Is there any way to normalized data for DE step based on their library size?