Question: How to extract strain genome sequence from mouse gut microbiome metagenome shotgun sequence?
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gravatar for lxu12
2.7 years ago by
lxu120
lxu120 wrote:

Is there any tool to extract a specific strain genome from mouse gut microbiome shotgun metagenome sequence?

genome • 675 views
ADD COMMENTlink modified 2.7 years ago by h.mon29k • written 2.7 years ago by lxu120
1
gravatar for h.mon
2.7 years ago by
h.mon29k
Brazil
h.mon29k wrote:

If you have the sequence of the desired strain, you can do this with BBDuk.

ADD COMMENTlink written 2.7 years ago by h.mon29k

Thank you for your answer.

From heatmap and merged_abundance_table, I am interested in one strain or species. If I would check whether there is variant in the metagenome in a specific strain or species before and after treatment in mouse gut microbiome, are there any else tools? In this case, whether variant calling can be used directly, such as BWA-picard-GATK? If strain, what reference could be? Whether I can use a specific strain genome as reference? And if species, what reference could be? Many thanks.

ADD REPLYlink written 2.7 years ago by lxu120
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