A simple question about the differential expression. When I analyse the differential expression and heatmap, I was confused about the Log2(FPKM) and log2foldchange. I only know Log2(FPKM) is a method of normalization, and it can used for building heatmap, but what the function about log2foldchange? Only uesd for knowing which data was up-regulation or down-regulation? but, Log2(FPKM) also can it.
Supposing one data that control:0.04, drought 1h:1.73, drought 6h:4.15. But the heatmap shows that the all the differential datas range from 0 to 10. What's the difinition about up-regulation/down-regulation? Is drought/control >2 consider as up-regulation or the data which greater than 5 ?
You'll need a statistical test to determine if something is up or down regulated. Fold change cut off is not a good threshold for this.
I suggest you use limma or edgeR for this.