NOISEq object not contsructing properly

Question

[manu.tiwari]

Hello all

I am using NOISeq for analysis and am facing a problem when making the NOISeq object.

I have separate csv files with the following column names to be used for additional biological annotation:

Enseml_ID GC content

,

Ensembl_ID Biotype

,

Enseml_ID Chromosome Start Position End Position

and others.

However, When I try making the NOISeq object as in the vignette, I get the following error:

Error in readData(data = allCounts, length = mylength, gc = mygc, biotype = mybiotypes,  : 
  The chromosome object should be a matrix or data.frame with 3 columns: chromosome, start position and end position.

Maybe it is a simple R issue but being new to R, I would greatly appreciate if you could elaborate on how to construct the object. Of course I can use only 3 columns corresponding to chromosome, start position and end position but then I will l ose the Enseml_IDs.

M

[h.mon]

It is impossible for anyone to help as you did not show how you tried to load your data, nor your data itself. The error message is clear, your chromosome object does not conform to what is expected. Can you paste here the output of head( x ), where x is your chromosome data?