Question: problem with tRNAseq alignment
2.4 years ago by
alirezamomeni707 • 0
alirezamomeni707 • 0 wrote:
I am trying to align tRNAseq data to the tRNA transcriptom. I made the index files using bowtie and trying to align using tophat. when I run the program for a single file I got this error:
Warning: Empty fasta file: 'tophat_out/tmp/segment_juncs.fa' Warning: All fasta inputs were empty Error: Encountered internal Bowtie 2 exception (#1) Command: bowtie2-build --wrapper basic-0 tophat_out/tmp/segment_juncs.fa tophat_out/tmp/segment_juncs [FAILED] Error: Splice sequence indexing failed with err =1
do you know how solve this problem?
ADD COMMENT • link •