RepeatModeler and RepeatMasker for non-model species

Question

[nancydong20]

Hello everyone!

I am trying to analyze repeat elements in a new draft genome of a non-model organism. I used RepeatModeler to build a de novo repeat library, and then used RepeatMasker (rmblastn version 2.2.27+) to mask the genome. In the .tbl output, I see that no simple repeats or low complexity regions were annotated and many of the subfamilies of SINEs and LINEs are 0%.

An image of the output can be see here: https://ibb.co/djErSF

The commands I used were:

> BuildDatabase -name Reference -engine ncbi ref.fa
> RepeatModeler -engine ncbi -database Reference  
> RepeatMasker -s -lib ~/consensi.fa.classified -dir ~/my_RM_output_dir_Aug11 -e ncbi ~/database/LS_genome.fa

If this is useful in anyway, the query species was assumed to be fly and the analysis used the RepeatMasker Combined Database: Dfam_Consensus-20170127, RepBase-20170127.

What could be the reason that many of the results are coming back as 0%?

Thank you very much!

[ashaneev07]

Did u solve ur problem.? because i'm also getting something similar to ur result.