Question: Multiple sequence alignement MSA editing
0
gravatar for TEman
24 months ago by
TEman10
Sweden
TEman10 wrote:

I want to remove all rare insertions (when it occurs in less than 5% of the sequences) in a multiple sequence alignment file (clustal .aln) with 699 sequences.

That is, I have a MSA with many columns containing only one or two insertions while the rest of the sequences are blank "-". It is by far too much to do manually.

Any suggestions how to do this?

alignment clustal R • 618 views
ADD COMMENTlink written 24 months ago by TEman10
1

Do you specifically want to do this in R?

If you use BioPython, you can create an ungapped concensus sequence with a threshold for inclusion of a particular residue in a column.

ADD REPLYlink written 24 months ago by jrj.healey13k
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