Question: species relative abundance
0
gravatar for a.alsayed.phd
8 months ago by
a.alsayed.phd10 wrote:

Hello, I have OTUs (from 16 s microbiome analysis - MIseq) on excel sheets for many samples. I would like to plot the relative abundance of each species in a graph. I am new to this field, so please, can anyone advise me how to do that? Many Thanks

16s • 555 views
ADD COMMENTlink modified 8 months ago by Brian Bushnell15k • written 8 months ago by a.alsayed.phd10

This is a question, not a tool post, so I have updated your post.

But it's not clear from your question whether you are trying to calculate the relative abundance from reads, or whether you already know the abundance and are just trying to plot it. Can you clarify?

ADD REPLYlink written 8 months ago by Brian Bushnell15k

Thank you for your reply I laready know the abundance for each OTU and I am trying to know the relative abundance and plot it.

ADD REPLYlink written 8 months ago by a.alsayed.phd10

If you already have the abundance data in Excel... then this is not really a bioinformatics question. Generally, you go to the "Insert" tab, draw a rectangle around the data, select "Column" and pick a chart type.

Try this for more information:

https://blog.hubspot.com/marketing/how-to-build-excel-graph

ADD REPLYlink written 8 months ago by Brian Bushnell15k

Thank you for your advise I know, but I have around 137 rows, 3 examples (OTU ID) as following: k__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Streptococcus k__Bacteria;p__Actinobacteria;c__Actinobacteria;o__Actinomycetales;f__Micrococcaceae;g__Rothia k__Bacteria;p__Fusobacteria;c__Fusobacteriia;o__Fusobacteriales;f__Fusobacteriaceae;g__Fusobacterium

and I am not sure how to compare each species individually? so not the combinations comparison

ADD REPLYlink written 8 months ago by a.alsayed.phd10

This sounds like a problem needing a customized solution. I think you need to talk to the bioinformatician working with you...

ADD REPLYlink written 8 months ago by Brian Bushnell15k
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