I would be interested to know if there is a publication or other reference for NGS statistics.
The NGS guidelines published in 2016 (PMID: 26508566) provide an estimate of probability to miss a variant in different situations, namely considering the % of bases covered >20x. Eg. the following situations 75% of target bases >20x, 86% >20x, 96% >20x.
I was wondering if there are other relevant articles to read and what would be the expected percentage of bases >20X for a 65X and 130X (mean coverage) exome.
Thank you in advance!