I am analyzing RNA seq data and trying to identify differentially expressed genes between to populations using ttest (my data is in triplicates)
I was wondering weather I need to check for the variance assumptions before applying ttest.
if so- what test will be recommended to use on such data?
I have tried Bartlett's, Levene's (with absolute values), Levene's (with quadratic values), Brown-Forsythe and two-sample F-test
and got different though close results. I would live to hear your thoughts! Tnx!