Question: What is the orientation of the sequences in illumina paired end output file?
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gravatar for Shanika
2.3 years ago by
Shanika0
Shanika0 wrote:

In a illumina paired end sequencing output file, are both the two corresponding sequences in the same orientation (like both are in 5' to 3') or are they in different orientaions (like one in 5' to 3' and other one in 3' to 5')?

paired end next-gen illumina • 1.1k views
ADD COMMENTlink written 2.3 years ago by Shanika0
1

Sequence reads are always in 5'-->3' orientation. See: Orientation in paired-end sequencing? . Mappers will automatically reverse complement reads (as needed) when mapping the reads.

ADD REPLYlink modified 2.3 years ago • written 2.3 years ago by genomax78k
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