How to find common alternative splicing events or novel transcripts among different batches of samples?
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3.4 years ago
wenhui • 0

Hello,

Recently, I am interested in finding common mutation among different batches of samples. But I have not found approaches to determine common alternative splicing and novel transcripts. Any advice about this topic is appreciated. Thank you.

RNA-Seq splicing novel transcript • 1.9k views
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3.4 years ago

I believe that the HISAT2 / StringTie pipeline is suited for this. This was originally TopHat2 / Cufflinks.

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3.4 years ago

The pipeline Kevin suggest will give you the basis for doing the downstream analysis of acutal splice events.

If you are interested in isoform switches (which enables easy biological interpretation) you can use one of my tool IsoformSwitchAnalyzeR. Alternatively rMATS or DEXSeq can be used to find exon-level differences. Leafcutter lies somewhere in between analyzing clusters of splicing.

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The pipeline Kevin suggest will give you the basis for doing the downstream analysis of acutal splice events.

Thanks Kristoffer, that is certainly true, and I also recommend the programs that you have mentioned.

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