Question: Possible to directly compare RNA-Seq and DGE data?
1
gravatar for ez935
16 months ago by
ez93510
ez93510 wrote:

Hello,

I have sequencing data from RNA-Seq and DGE (Digital Gene Expression) for the following conditions:

RNA-Seq: Condition A + Condition B

DGE: Condition B + Condition C

Would it be possible to compare Condition A against Condition C from different methods? These experiments were done at different times so we no longer have the samples available to run them on the same platform.

Thanks!

dge rna-seq • 670 views
ADD COMMENTlink modified 16 months ago • written 16 months ago by ez93510
2

To add to Sean Davis : No, library prep/quantification methods will seriously bias the result. You would be doing a technical comparison of the method RNA-seq with the method DGE, and not the underlying biological comparison of condition A vs condition C.

ADD REPLYlink modified 16 months ago • written 16 months ago by WouterDeCoster38k
2
gravatar for Sean Davis
16 months ago by
Sean Davis25k
National Institutes of Health, Bethesda, MD
Sean Davis25k wrote:

Unfortunately, probably not.

ADD COMMENTlink written 16 months ago by Sean Davis25k
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